A conserved core of programmed cell death indicator genes discriminates developmentally and environmentally induced programmed cell death in plants Journal Article


Author(s): Olvera-Carrillo, Yadira; Van Bel, Michiel; Van Hautegem, Tom; Fendrych, Matyas; Huysmans, Marlies; Šimášková, Mária; Van Durme, Matthias; Buscaill, Pierre; Rivas, Susana; Coll, Núria S; Coppens, Frederik; Maere, Steven; Nowack, Moritz K
Article Title: A conserved core of programmed cell death indicator genes discriminates developmentally and environmentally induced programmed cell death in plants
Affiliation IST Austria
Abstract: A plethora of diverse programmed cell death (PCD) processes has been described in living organisms. In animals and plants, different forms of PCD play crucial roles in development, immunity, and responses to the environment. While the molecular control of some animal PCD forms such as apoptosis is known in great detail, we still know comparatively little about the regulation of the diverse types of plant PCD. In part, this deficiency in molecular understanding is caused by the lack of reliable reporters to detect PCD processes. Here, we addressed this issue by using a combination of bioinformatics approaches to identify commonly regulated genes during diverse plant PCD processes in Arabidopsis (Arabidopsis thaliana). Our results indicate that the transcriptional signatures of developmentally controlled cell death are largely distinct from the ones associated with environmentally induced cell death. Moreover, different cases of developmental PCD share a set of cell death-associated genes. Most of these genes are evolutionary conserved within the green plant lineage, arguing for an evolutionary conserved core machinery of developmental PCD. Based on this information, we established an array of specific promoter-reporter lines for developmental PCD in Arabidopsis. These PCD indicators represent a powerful resource that can be used in addition to established morphological and biochemical methods to detect and analyze PCD processes in vivo and in planta.
Journal Title: Plant Physiology
Volume: 169
Issue 4
ISSN: 1532-2548
Publisher: American Society of Plant Biologists  
Date Published: 2015-12-01
Start Page: 2684
End Page: 2699
Sponsor: This work was supported by the Consejo Nacional de Ciencia y Tecnología (postdoctoral fellowship registration nos. 186253 and 203288 to Y.O.-C.) and the French Laboratory of Excellence (project TULIP ANR–10–LABX–41 and ANR–11–IDEX–0002–02).
DOI: 10.1104/pp.15.00769
Notes: We thank all members of the PCD research team at the Vlaams Instituut voor Biotechnologie-Plant Systems Biology Department department for critical reading of the article, Annick Bleys for help in revising the cited references, other members of the Vlaams Instituut voor Biotechnologie-Plant Systems Biology Department for sharing fields of expertise, Dr. Marc Heijde and Dr. Toon Cools for the genotoxic stress experiments, and Dr. Pavel Kerchev for the oxidative stress experiments.
Open access: no