Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton Journal Article


Author(s): Toshima, Junko Y; Furuya, Eri; Nagano, Makoto; Kanno, Chisa; Sakamoto, Yuta; Ebihara, Masashi; Siekhaus, Daria E; Toshima, Jiro
Article Title: Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton
Affiliation IST Austria
Abstract: The actin cytoskeleton plays important roles in the formation and internalization of endocytic vesicles. In yeast, endocytic vesicles move towards early endosomes along actin cables, however, the molecular machinery regulating interaction between endocytic vesicles and actin cables is poorly understood. The Eps15-like protein Pan1p plays a key role in actin-mediated endocytosis and is negatively regulated by Ark1 and Prk1 kinases. Here we show that pan1 mutated to prevent phosphorylation at all 18 threonines, pan1-18TA, displayed almost the same endocytic defect as ark1Δ prk1Δ cells, and contained abnormal actin concentrations including several endocytic compartments. Early endosomes were highly localized in the actin concentrations and displayed movement along actin cables. The dephosphorylated form of Pan1p also caused stable associations between endocytic vesicles and actin cables, and between endocytic vesicles and endosomes. Thus Pan1 phosphorylation is part of a novel mechanism that regulates endocytic compartment interactions with each other and with actin cables.
Keywords: Endocytosis; Cell biology; actin; Endosome; Clathrin
Journal Title: eLife
Volume: 2016
Issue 5
ISSN: 2050-084X
Publisher: eLife Sciences Publications  
Date Published: 2016-04-04
Start Page: Article number: e10276
Copyright Statement: CC BY 4.0
Sponsor: Daria E Siekhaus is supported by EU grant PCIG12-GA-2012-334077.
URL:
DOI: 10.7554/eLife.10276.002
Open access: yes (OA journal)