PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis Journal Article

Author(s): Simon, Sibu; Skůpa, Petr; Viaene, Tom; Zwiewka, Marta; Tejos, Ricardo; Klíma, Petr; Čarná, Mária; Rolčík, Jakub; De Rycke, Riet M; Moreno, Ignacio; Dobrev, Petre Ivanov; Orellana, Ariel; Zažímalová, Eva; Friml, Jiří
Article Title: PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis
Affiliation IST Austria
Abstract: Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
Keywords: Lateral root; Auxin; PIN; Endoplasmic reticulum (ER); Plasma membrane (PM)
Journal Title: New Phytologist
Volume: 211
Issue 1
ISSN: 1469-8137
Publisher: Wiley-Blackwell  
Date Published: 2016-07-01
Start Page: 65
End Page: 74
DOI: 10.1111/nph.14019
Notes: This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP, project CEITEC (CZ.1.05/1.1.00/02.0068) and the Czech Science Foundation GA  CR (project no. 13-4063 7S to J.F. and 16-10948S to E.Z.); FONDAP-CRG 15090007 and Basal PB16 (to A.O.). The authors thank Enrico Scarpella and Clay Carter for kindly providing published materials; Klara Hoyerova, Marleen Vanstrae- len and Zhaojun Din g for helpful discussion and help with experiments.
Open access: yes (repository)