Spatiotemporal microbial evolution on antibiotic landscapes Journal Article


Author(s): Baym, Michael; Lieberman, Tami D; Kelsic, Eric D; Chait, Remy; Gross, Rotem; Yelin, Idan; Kishony, Roy K
Article Title: Spatiotemporal microbial evolution on antibiotic landscapes
Alternate Title: Science
Affiliation IST Austria
Abstract: A key aspect of bacterial survival is the ability to evolve while migrating across spatially varying environmental challenges. Laboratory experiments, however, often study evolution in well-mixed systems. Here, we introduce an experimental device, the microbial evolution and growth arena (MEGA)-plate, in which bacteria spread and evolved on a large antibiotic landscape (120 × 60 centimeters) that allowed visual observation of mutation and selection in a migrating bacterial front.While resistance increased consistently, multiple coexisting lineages diversified both phenotypically and genotypically. Analyzing mutants at and behind the propagating front,we found that evolution is not always led by the most resistant mutants; highly resistant mutants may be trapped behindmore sensitive lineages.TheMEGA-plate provides a versatile platformfor studying microbial adaption and directly visualizing evolutionary dynamics.
Keywords: Bacteria (microorganisms)
Journal Title: Science
Volume: 353
Issue 6304
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2016-09-09
Start Page: 1147
End Page: 1151
Sponsor: Supported by National Defense Science and Engineering Graduate fellowship 32 CFR 168a (E.D.K.), NIH grant R01-GM081617 (R.K.), and European Research Council FP7 ERC grant 281891 (R.K.).
DOI: 10.1126/science.aag0822
Notes: J.R.F. thanks NIH (grant HL034300, HL111392, DK038226) and the Robert A. Welch Foundation (grant I-0011) for funding. L.K. gratefully acknowledges the generous financial support of Rice University, NIH (grant R01 GM-114609-01), NSF (CAREER:SusChEM CHE-1455335), the Robert A. Welch Foundation (grant C-1764), American Chemical Society Petroleum Research Fund (grant 51707-DNI1), Amgen (2014 Young Investigators ’ Award to L.K.), and Biotage (2015 Young Principal Investigator Award). A provisional patent application (patent no. 62/360,859) has been submitted and assigned jointly to University of Texas Southwestern and Rice University Supported by National Defense Science and Engineering Graduate fellowship 32 CFR 168a (E.D.K.), NIH grant R01-GM081617 (R.K.), and European Research Council FP7 ERC grant 281891 (R.K.).
Open access: no