Pervasive selection for and against antibiotic resistance in inhomogeneous multistress environments Journal Article

Author(s): Chait, Remy; Palmer, Adam C; Yelin, Idan; Kishony, Roy
Article Title: Pervasive selection for and against antibiotic resistance in inhomogeneous multistress environments
Affiliation IST Austria
Abstract: Antibiotic-sensitive and -resistant bacteria coexist in natural environments with low, if detectable, antibiotic concentrations. Except possibly around localized antibiotic sources, where resistance can provide a strong advantage, bacterial fitness is dominated by stresses unaffected by resistance to the antibiotic. How do such mixed and heterogeneous conditions influence the selective advantage or disadvantage of antibiotic resistance? Here we find that sub-inhibitory levels of tetracyclines potentiate selection for or against tetracycline resistance around localized sources of almost any toxin or stress. Furthermore, certain stresses generate alternating rings of selection for and against resistance around a localized source of the antibiotic. In these conditions, localized antibiotic sources, even at high strengths, can actually produce a net selection against resistance to the antibiotic. Our results show that interactions between the effects of an antibiotic and other stresses in inhomogeneous environments can generate pervasive, complex patterns of selection both for and against antibiotic resistance.
Keywords: nonhuman; article; fitness; inhibition; Bacteria (microorganisms); chloramphenicol; ciprofloxacin; doxycycline; rifampicin; cefoxitin; cotrimoxazole; nitrofurantoin; spectinomycin; spiramycin; tetracycline; tigecycline; tobramycin; bacterium; environmental stress; toxin; sensitivity; drug potentiation; physical stress; cefoxitin, 33564-30-6, 35607-66-0; chloramphenicol, 134-90-7, 2787-09-9, 56-75-7; ciprofloxacin, 85721-33-1; cotrimoxazole, 8064-90-2; doxycycline, 10592-13-9, 17086-28-1, 564-25-0, 94088-85-4; nitrofurantoin, 54-87-5, 67-20-9; rifampicin, 13292-46-1; spectinomycin, 1695-77-8, 21736-83-4, 23312-56-3; spiramycin, 8025-81-8; tetracycline, 23843-90-5, 60-54-8, 64-75-5, 8021-86-1; tigecycline, 220620-09-7; tobramycin, 32986-56-4
Journal Title: Nature Communications
Volume: 7
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2016-01-20
Start Page: Article number: 10333
Copyright Statement: CC-BY
DOI: 10.1038/ncomms10333
Notes: We are grateful to Michael Baym, Michael Elowitz, Morten Ernebjerg, Oshri Halimi, Ylaine Gerardin, Dan Kahne, Yifat Merbl, Jean-Baptiste Michel, Georg Rieckh, Aakash Shah, Noam Shoresh, Shreya Shrestha, Laura Stone, Nicholas Stroustrup, Tami Lieberman and Kalin Vetsigian for helpful discussion, comments and suggestions, and Andreas Angermayr, Tobias Bollenbach, Daniel Schultz and Gertraude Stift for technical assistance. This work was partially supported by US National Institutes of Health grant R01-GM081617, Israeli Centers of Research Excellence I-CORE Program ISF Grant No. 152/11, and the European Research Council FP7 ERC Grant 281891.
Open access: yes (OA journal)