Optogenetic control of fly optomotor responses Journal Article


Author(s): Haikala, Väinö; Jösch, Maximilian; Borst, Alexander; Mauss, Alex S
Article Title: Optogenetic control of fly optomotor responses
Affiliation
Abstract: When confronted with a large-field stimulus rotating around the vertical body axis, flies display a following behavior called "optomotor response." As neural control elements, the large tangential horizontal system (HS) cells of the lobula plate have been prime candidates for long. Here, we applied optogenetic stimulation of HS cells to evaluate their behavioral role in Drosophila. To minimize interference of the optical activation of channelrhodopsin-2 with the visual perception of the flies, we used a bistable variant called ChR2-C128S. By applying pulses of blue and yellow light, we first demonstrate electrophysiologically that lobula plate tangential cells can be activated and deactivated repeatedly with no evident change in depolarization strength over trials. We next show that selective optogenetic activation of HS cells elicits robust yaw head movements and yaw turning responses in fixed and tethered flying flies, respectively.
Keywords: Drosophila; rhodopsin; Confocal microscopy; Diptera; brain electrophysiology; depolarization
Journal Title: Journal of Neuroscience
Volume: 33
Issue 34
ISSN: 1529-2401
Publisher: Society for Neuroscience  
Date Published: 2013-01-01
Start Page: 13927
End Page: 13934
Sponsor: This work was supported by the Max Planck Society.
DOI: 10.1523/JNEUROSCI.0340-13.2013
Open access: no