Endosomal interactions during root hair growth Journal Article


Author(s): von Wangenheim, Daniel; Rosero, Amparo; Komis, George; Šamajová, Olga; Ovečka, Miroslav; Voigt, Boris; Šamaj, Jozef
Article Title: Endosomal interactions during root hair growth
Affiliation IST Austria
Abstract: The dynamic localization of endosomal compartments labeled with targeted fluorescent protein tags is routinely followed by time lapse fluorescence microscopy approaches and single particle tracking algorithms. In this way trajectories of individual endosomes can be mapped and linked to physiological processes as cell growth. However, other aspects of dynamic behavior including endosomal interactions are difficult to follow in this manner. Therefore, we characterized the localization and dynamic properties of early and late endosomes throughout the entire course of root hair formation by means of spinning disc time lapse imaging and post-acquisition automated multitracking and quantitative analysis. Our results show differential motile behavior of early and late endosomes and interactions of late endosomes that may be specified to particular root hair domains. Detailed data analysis revealed a particular transient interaction between late endosomes—termed herein as dancing-endosomes—which is not concluding to vesicular fusion. Endosomes preferentially located in the root hair tip interacted as dancing-endosomes and traveled short distances during this interaction. Finally, sizes of early and late endosomes were addressed by means of super-resolution structured illumination microscopy (SIM) to corroborate measurements on the spinning disc. This is a first study providing quantitative microscopic data on dynamic spatio-temporal interactions of endosomes during root hair tip growth.
Keywords: endosomes; Development; Arabidopsis thaliana; Trafficking; Root hair; Interaction; Spinning disc microscopy; Structured illumination microscopy
Journal Title: Frontiers in Plant Science
Volume: 6
Issue JAN2016
ISSN: 1664-462X
Publisher: Frontiers Research Foundation  
Date Published: 2016-01-29
Start Page: Article number: 1262
Copyright Statement: CC BY 4.0
Sponsor: This work was supported by National Program for Sustainability I (grant no. LO1204) provided by the Czech Ministry of Education and by Institutional Fund of Palacký University Olomouc (GK and OŠ).
URL:
DOI: 10.3389/fpls.2015.01262
Notes: We thank Sabine Fischer for help with the statistics.
Open access: yes (OA journal)