Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein Journal Article


Author(s): Michalko, Jaroslav; Glanc, Matous; Perrot-Rechenmann, Catherine; Friml, Jiří
Article Title: Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein
Affiliation IST Austria
Abstract: The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants ( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles.
Journal Title: F1000 Research
Volume: 5
ISSN: 2046-1402
Publisher: F1000 Research Ltd.  
Date Published: 2016-01-01
Start Page: Article number: 86
Copyright Statement: CC BY 4.0
URL:
DOI: 10.12688/f1000research.7654.1
Notes: This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG was supported by the scholarship "Stipendien der Stipendienstiftung der Republik Österreich". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We would like to thank Mark Estelle and Yunde Zhao for provid - ing abp1-c1 , abp1-TD1 and abp1-WTc1 seeds. We thank Emeline Huault for technical assistance.
Open access: yes (OA journal)