Comparative genomic analysis of Haemophilus haemolyticus and nontypeable Haemophilus influenzae and a new testing scheme for their discrimination Journal Article


Author(s): Hu, Fang; Rishishwar, Lavanya; Sivadas, Ambily; Mitchell, Gabriel J; King, Jordan I; Murphy, Timothy F; Gilsdorf, Janet R; Mayer, Leonard W; Wang, Xin
Article Title: Comparative genomic analysis of Haemophilus haemolyticus and nontypeable Haemophilus influenzae and a new testing scheme for their discrimination
Affiliation IST Austria
Abstract: Haemophilus haemolyticus has been recently discovered to have the potential to cause invasive disease. It is closely related to nontypeable Haemophilus influenzae (NT H. influenzae). NT H. influenzae and H. haemolyticus are often misidentified because none of the existing tests targeting the known phenotypes of H. haemolyticus are able to specifically identify H. haemolyticus. Through comparative genomic analysis of H. haemolyticus and NT H. influenzae, we identified genes unique to H. haemolyticus that can be used as targets for the identification of H. haemolyticus. A real-time PCR targeting purT (encoding phosphoribosylglycinamide formyltransferase 2 in the purine synthesis pathway) was developed and evaluated. The lower limit of detection was 40 genomes/PCR; the sensitivity and specificity in detecting H. haemolyticus were 98.9% and 97%, respectively. To improve the discrimination of H. haemolyticus and NT H. influenzae, a testing scheme combining two targets (H. haemolyticus purT and H. influenzae hpd, encoding protein D lipoprotein) was also evaluated and showed 96.7% sensitivity and 98.2% specificity for the identification of H. haemolyticus and 92.8% sensitivity and 100% specificity for the identification of H. influenzae, respectively. The dual-target testing scheme can be used for the diagnosis and surveillance of infection and disease caused by H. haemolyticus and NT H. influenzae.
Journal Title: Journal of Clinical Microbiology
Volume: 54
Issue 12
ISSN: 00951137
Publisher: American Society for Microbiology  
Date Published: 2016-12-01
Start Page: 3010
End Page: 3017
Sponsor: We are grateful to ABCs for providing strains and the Bacterial Meningitis Laboratory for technical support.
URL:
DOI: 10.1128/JCM.01511-16
Open access: yes (repository)