Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development Journal Article


Author(s): Cucinotta, Mara; Manrique, Silvia; Guazzotti, Andrea; Quadrelli, Nadia E; Mendes, Marta A M; Benková, Eva; Colombo, Lucia
Article Title: Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development
Affiliation IST Austria
Abstract: The developmental programme of the pistil is under the control of both auxin and cytokinin. Crosstalk between these factors converges on regulation of the auxin carrier PIN-FORMED 1 (PIN1). Here, we show that in the triple transcription factor mutant cytokinin response factor 2 (crf2) crf3 crf6 both pistil length and ovule number were reduced. PIN1 expression was also lower in the triple mutant and the phenotypes could not be rescued by exogenous cytokinin application. pin1 complementation studies using genomic PIN1 constructs showed that the pistil phenotypes were only rescued when the PCRE1 domain, to which CRFs bind, was present. Without this domain, pin mutants resemble the crf2 crf3 crf6 triple mutant, indicating the pivotal role of CRFs in auxin-cytokinin crosstalk.
Keywords: Plant hormones; PIN1; CRFs; Ovule primordia; Pistil
Journal Title: Development
Volume: 143
Issue 23
ISSN: 1477-9129
Publisher: Company of Biologists  
Date Published: 2016-12-01
Start Page: 4419
End Page: 4424
DOI: 10.1242/dev.143545
Notes: M.C. was funded by a PhD fellowship from the Università degli Studi di Milano-Bicocca and from Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR) [MIUR-PRIN 2012]. L.C. is also supported by MIUR [MIUR-PRIN 2012]. We would like to thank Andrew MacCabe and Edward Kiegle for editing the paper.
Open access: no