A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients Journal Article

Author(s): Schwarz, Jan; Bierbaum, Veronika; Merrin, Jack; Frank, Tino; Hauschild, Robert; Bollenbach, Tobias; Tay, Savaş; Sixt, Michael; Mehling, Matthias
Article Title: A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients
Affiliation IST Austria
Abstract: Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue.
Journal Title: Scientific Reports
Volume: 6
ISSN: 20452322
Publisher: Nature Publishing Group  
Date Published: 2016-11-01
Start Page: Article number: 36440
Copyright Statement: CC BY
DOI: 10.1038/srep36440
Notes: This work was supported by the Swiss National Science Foundation (Ambizione fellowship; PZ00P3-154733 to M.M.), the Swiss Multiple Sclerosis Society (research support to M.M.), a fellowship from the Boehringer Ingelheim Fonds (BIF) to J.S., the European Research Council (grant ERC GA 281556) and a START award from the Austrian Science Foundation (FWF) to M.S. #BioimagingFacility
Open access: yes (OA journal)