Microtubule aging probed by microfluidics assisted tubulin washout Journal Article


Author(s): Duellberg, Christian; Cade, Nicholas I; Surrey, Thomas
Article Title: Microtubule aging probed by microfluidics assisted tubulin washout
Affiliation
Abstract: Microtubules switch stochastically between phases of growth and shrinkage. The molecular mechanism responsible for the end of a growth phase, an event called catastrophe, is still not understood. The probability for a catastrophe to occur increases with microtubule age, putting constraints on the possible molecular mechanism of catastrophe induction. Here we used microfluidics-Assisted fast tubulin washout experiments to induce microtubule depolymerization in a controlled manner at different times after the start of growth. We found that aging can also be observed in this assay, providing valuable new constraints against which theoretical models of catastrophe induction can be tested. We found that the data can be quantitatively well explained by a simple kinetic threshold model that assumes an age-dependent broadening of the protective cap at the microtubule end as a result of an evolving tapered end structure; this leads to a decrease of the cap density and its stability. This analysis suggests an intuitive picture of the role of morphological changes of the protective cap for the age dependence of microtubule stability.
Journal Title: Molecular Biology and Evolution
Volume: 27
Issue 22
ISSN: 0737-4038
Publisher: OUP  
Date Published: 2016-11-07
Start Page: 3563
End Page: 3573
DOI: 10.1091/mbc.E16-07-0548
Open access: no