Flavonol induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1 2 mutant require phosphatase activity Journal Article


Author(s): Kuhn, Benjamin M; Nodzyński, Tomasz; Errafi, Sanae; Bucher, Rahel; Gupta, Shibu; Aryal, Bibek; Dobrev, Petre; Bigler, Laurent; Geisler, Markus; Zažímalová, Eva; Friml, Jiří; Ringli, Christoph
Article Title: Flavonol induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1 2 mutant require phosphatase activity
Affiliation IST Austria
Abstract: The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in flavonol accumulation in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants show wild type-like auxin transport activity while levels of free auxin are not affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization. In vivo analysis of PINOID action, a kinase known to influence PIN protein localization in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID activity. Together, these data suggest that flavonols affect auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.
Keywords: Cell growth, developmental biology, plant sciences
Journal Title: Scientific Reports
Volume: 7
ISSN: 20452322
Publisher: Nature Publishing Group  
Date Published: 2017-02-06
Start Page: Article number: 41906
URL:
DOI: 10.1038/srep41906
Notes: This work was supported by the Forschungskredit of the University of Zurich [BMK] and the Swiss National Science Foundation Grant No. 3100A0–122157 [BMK], 3100A0–122157 and 31003A-138472 [CR, SE], and 31003A_144223 [MG]; Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601) and project Postdoc I. (CZ.1.07/2.3.00/30.0009) [TN]; the Czech Science Foundation (GA16-10948S) [PD, EZ]; European Research Council (project ERC-2011-StG-20101109-PSDP), European Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science Foundation (GA13-40637S) [JF].
Open access: yes (OA journal)