Insulation and wiring specificity of BceR like response regulators and their target promoters in Bacillus subtilis Journal Article


Author(s): Fang, Chong; Nagy-Staron, Anna; Grafe, Martin; Heermann, Ralf; Jung, Kirsten; Gebhard, Susanne; Mascher, Thorsten
Article Title: Insulation and wiring specificity of BceR like response regulators and their target promoters in Bacillus subtilis
Affiliation IST Austria
Abstract: BceRS and PsdRS are paralogous two-component systems in Bacillus subtilis controlling the response to antimicrobial peptides. In the presence of extracellular bacitracin and nisin, respectively, the two response regulators (RRs) bind their target promoters, PbceA or PpsdA, resulting in a strong up-regulation of target gene expression and ultimately antibiotic resistance. Despite high sequence similarity between the RRs BceR and PsdR and their known binding sites, no cross-regulation has been observed between them. We therefore investigated the specificity determinants of PbceA and PpsdA that ensure the insulation of these two paralogous pathways at the RR–promoter interface. In vivo and in vitro analyses demonstrate that the regulatory regions within these two promoters contain three important elements: in addition to the known (main) binding site, we identified a linker region and a secondary binding site that are crucial for functionality. Initial binding to the high-affinity, low-specificity main binding site is a prerequisite for the subsequent highly specific binding of a second RR dimer to the low-affinity secondary binding site. In addition to this hierarchical cooperative binding, discrimination requires a competition of the two RRs for their respective binding site mediated by only slight differences in binding affinities.
Journal Title: Molecular Microbiology
Volume: 104
Issue 1
ISSN: 1365-2958
Publisher: Wiley-Blackwell  
Date Published: 2017-04-01
Start Page: 16
End Page: 31
DOI: 10.1111/mmi.13597
Notes: The authors would like to thank Ainhoa Revilla Guarinos for critical reading of the manuscript and Julia Frunzke for the gift of synthetic phosphoramidate. This work was supported by a grant from the Deutsche Forschungsgemeinschaft (DFG; grant MA2837/1-3 to T.M., and Exc114/2 to K.J.). C.F. was supported by a PhD scholarship from the China Scholarship Council. SPR experiments were performed in the Bioanalytics core facility of the LMU Biocenter.
Open access: no