The physical basis of coordinated tissue spreading in zebrafish gastrulation Journal Article


Author(s): Morita, Hitoshi; Grigolon, Silvia; Bock, Martin; Krens, Gabriel; Salbreux, Guillaume; Heisenberg, Carl-Philipp
Article Title: The physical basis of coordinated tissue spreading in zebrafish gastrulation
Affiliation IST Austria
Abstract: Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during “doming,” when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.
Keywords: Gastrulation; Zebrafish; Active fluid description of tissue spreading; radial cell intercalation; surface cell expansion; interfacial tension
Journal Title: Developmental Cell
Volume: 40
Issue 4
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2017-02-27
Start Page: 354
End Page: 366
Copyright Statement: CC BY 4.0
URL:
DOI: 10.1016/j.devcel.2017.01.010
Notes: We thank O. Campàs, J.-F. Joanny, R. Mayor, P. Ziherl, and members of our laboratories for thoughtful discussions and comments on the manuscript, and the light microscopy and zebrafish facilities of the IST Austria for continuous support. This work was supported by postdoctoral fellowships from Uehara Memorial Foundation and JSPS to H.M., grants from IST Austria to C.-P.H., and the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317), to S.G. and G.S. #BioimagingFacility #LifeScienceFacility
Open access: yes (OA journal)