Green-light-induced inactivation of receptor signaling using cobalamin-binding domains Journal Article

Author(s): Kainrath, Stephanie; Stadler, Manuela; Reichhart, Eva; Distel, Martin; Janovjak, Harald
Article Title: Green-light-induced inactivation of receptor signaling using cobalamin-binding domains
Affiliation IST Austria
Abstract: Optogenetics and photopharmacology provide spatiotemporally precise control over protein interactions and protein function in cells and animals. Optogenetic methods that are sensitive to green light and can be used to break protein complexes are not broadly available but would enable multichromatic experiments with previously inaccessible biological targets. Herein, we repurposed cobalamin (vitamin B12) binding domains of bacterial CarH transcription factors for green-light-induced receptor dissociation. In cultured cells, we observed oligomerization-induced cell signaling for the fibroblast growth factor receptor 1 fused to cobalamin-binding domains in the dark that was rapidly eliminated upon illumination. In zebrafish embryos expressing fusion receptors, green light endowed control over aberrant fibroblast growth factor signaling during development. Green-light-induced domain dissociation and light-inactivated receptors will critically expand the optogenetic toolbox for control of biological processes.
Keywords: Optogenetics; receptors; photochromism; cobalamins
Journal Title: Angewandte Chemie - International Edition
Volume: 129
Issue 16
ISSN: 1521-3773
Publisher: Wiley-Blackwell  
Date Published: 2017-03-20
Start Page: 460
End Page: 4611
Sponsor: Austrian Science Fund. Grant Number: W1232 Österreichische Forschungsförderungsgesellschaft. Grant Number: 3580812 Seventh Framework Programme. Grant Number: CIG-303564
DOI: 10.1002/anie.201611998
Open access: yes (repository)