Optogenetic methods in drug screening: Technologies and applications Journal Article

Author(s): Agus, Viviana; Janovjak, Harald
Article Title: Optogenetic methods in drug screening: Technologies and applications
Affiliation IST Austria
Abstract: The optogenetic revolution enabled spatially-precise and temporally-precise control over protein function, signaling pathway activation, and animal behavior with tremendous success in the dissection of signaling networks and neural circuits. Very recently, optogenetic methods have been paired with optical reporters in novel drug screening platforms. In these all-optical platforms, light remotely activated ion channels and kinases thereby obviating the use of electrophysiology or reagents. Consequences were remarkable operational simplicity, throughput, and cost-effectiveness that culminated in the identification of new drug candidates. These blueprints for all-optical assays also revealed potential pitfalls and inspire all-optical variants of other screens, such as those that aim at better understanding dynamic drug action or orphan protein function.
Journal Title: Current Opinion in Biotechnology
Volume: 48
ISSN: 0958-1669
Publisher: Elsevier  
Date Published: 2017-12-01
Start Page: 8
End Page: 14
Sponsor: This work was supported by grants of the European Union Seventh Framework Programme (CIG-303564), the Human Frontier Science Program (RGY0084_2012), and the Austrian Science Fund FWF (W1232 MolecularDrugTargets).
DOI: 10.1016/j.copbio.2017.02.006
Open access: no