Live tracking of moving samples in confocal microscopy for vertically grown roots Journal Article


Author(s): von Wangenheim, Daniel; Hauschild, Robert; Fendrych, Matyas; Barone, Vanessa; Benková, Eva; Friml, Jiří
Article Title: Live tracking of moving samples in confocal microscopy for vertically grown roots
Affiliation IST Austria
Abstract: Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.
Keywords: Tracking; Gravitropism; Root; Confocal microscopy; time-lapse; gravistimulation
Journal Title: eLife
Volume: 6
ISSN: 2050-084X
Publisher: eLife Sciences Publications  
Date Published: 2017-06-19
Start Page: Article number: e26792
Copyright Statement: CC BY 4.0
URL:
DOI: 10.7554/eLife.26792
Notes: Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. The authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript. #BioimagingFacility
Open access: yes (OA journal)