Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows Journal Article

Author(s): Pinheiro, Diana; Hannezo, Edouard; Herszterg, Sophie; Bosveld, Floris; Gaugué, Isabelle; Balakireva, Maria G; Wang, Zhimin; Cristo, Inês; Rigaud, Stéphane U; Markova, Olga; Bellaïche, Yohanns
Article Title: Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows
Abstract: During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts between the dividing cell and its neighbours has profound implications for the integrity, arrangement and morphogenesis of proliferative tissues. In both vertebrates and invertebrates, this remodelling requires the activity of non-muscle myosin II (MyoII) in the interphasic cells neighbouring the dividing cell. However, the mechanisms that coordinate cytokinesis and MyoII activity in the neighbours are unknown. Here we show that in the Drosophila notum epithelium, each cell division is associated with a mechanosensing and transmission event that controls MyoII dynamics in neighbouring cells. We find that the ring pulling forces promote local junction elongation, which results in local E-cadherin dilution at the ingressing adherens junction. In turn, the reduction in E-cadherin concentration and the contractility of the neighbouring cells promote self-organized actomyosin flows, ultimately leading to accumulation of MyoII at the base of the ingressing junction. Although force transduction has been extensively studied in the context of adherens junction reinforcement to stabilize adhesive cell-cell contacts, we propose an alternative mechanosensing mechanism that coordinates actomyosin dynamics between epithelial cells and sustains the remodelling of the adherens junction in response to mechanical forces.
Journal Title: Nature
Volume: 545
Issue 7652
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2017-05-04
Start Page: 103
End Page: 107
DOI: 10.1038/nature22041
Notes: We thank M. Affolter, S. Bogdan, M. Fuller, J. Großhans, A. Martin, J. Mihaly, H. Oda, B. Sanson, D. St Johnston, J. Treisman, J. Zallen, VDRC, TRiP, Kyoto and Bloomington Stock Centres and DSHB for reagents; J. Prost, L. Szpiro for inputs; M. Thery, L. Blanchoin, Emilie Barou, H. Ennomanix, K. Cockburn and V. Greco for data and inputs; the Developmental Biology Unit imaging platform; J.-F. Joanny, F. Graner, A. Villedieu and R.-M. Mège for comments; P. Recho for help with simulations; ANR-MaxForce, ERC (TiMoprh, 340784), ARC (SL220130607097), ANR-DEEP (11-LBX-0044, ANR-10-IDEX-0001-02) and PSL grants for funding; FCT (SFRH/BD/51700/2011) and FRM (FDT20150531972) fellowships to D.P. and Wellcome Trust (110326/Z/15/Z), Trinity College and the Bettencourt-Schueller Foundation fellowships to E.H.
Open access: no