Physics of active jamming during collective cellular motion in a monolayer Journal Article


Author(s): García, Simón; Hannezo, Edouard; Elgeti, Jens; Joanny, Jean F; Silberzan, Pascal; Gov, Nir S
Article Title: Physics of active jamming during collective cellular motion in a monolayer
Affiliation
Abstract: Although collective cell motion plays an important role, for example during wound healing, embryogenesis, or cancer progression, the fundamental rules governing this motion are still not well understood, in particular at high cell density. We study here the motion of human bronchial epithelial cells within a monolayer, over long times. We observe that, as the monolayer ages, the cells slow down monotonously, while the velocity correlation length first increases as the cells slow down but eventually decreases at the slowest motions. By comparing experiments, analytic model, and detailed particle-based simulations, we shed light on this biological amorphous solidification process, demonstrating that the observed dynamics can be explained as a consequence of the combined maturation and strengthening of cell-cell and cell-substrate adhesions. Surprisingly, the increase of cell surface density due to proliferation is only secondary in this process. This analysis is confirmed with two other cell types. The very general relations between the mean cell velocity and velocity correlation lengths, which apply for aggregates of self-propelled particles, as well as motile cells, can possibly be used to discriminate between various parameter changes in vivo, from noninvasive microscopy data.
Keywords: Collective cell migration; Cell-cell adhesion; Dynamic inhomogeneity; Glass transition; Jamming
Journal Title: PNAS
Volume: 112
Issue 50
ISSN: 1091-6490
Publisher: National Academy of Sciences  
Date Published: 2015-12-15
Start Page: 15314
End Page: 15319
DOI: 10.1073/pnas.1510973112
Notes: We thank Isabelle Bonnet, Axel Buguin, Jacques Camonis, Fanny Cayrac, Sylvie Coscoy, Guillaume Duclos, Philippe Marcq, Maria Carla Parrini, and the members of the “biology-inspired physics at mesoscales” group for discussion and advice. We acknowledge the Cell and Tissue Imaging Platform (member of France-Bioimaging) of the Genetics and Developmental Biology Department (UMR3215/U934) of Institut Curie and, in particular, Olivier Renaud and Olivier Leroy. The laboratoire PhysicoChimie Curie is part of the Labex CelTisPhyBio. E.H. acknowledges the Bettencourt-Schueller Foundation's Young Researcher Prize and Trinity College's Junior Researcher Fellowship for their support. N.S.G. and P.S. thank the Kavli Institute for Theoretical Physics (KITP) at Santa Barbara for its hospitality during the Workshop on Active Matter. This research was supported in part by the National Science Foundation under Grant NSF PHY1125915 for the workshop program at KITP. N.S.G. acknowledges the Institut Curie’s Mayent-Rothschild Visiting Professor fund and Labex CelTisPhyBio for their support during the stay at the Institut Curie. N.S.G. is the incumbent of the Lee and William Abramowitz Professorial Chair of Biophysics, and thanks ISF Grant 580/12 for support. We are grateful to the Schmidt Minerva Center on Supramolecular Architecture for its support.
Open access: no