Assembly and positioning of actomyosin rings by contractility and planar cell polarity Journal Article


Author(s): Sehring, Ivonne M; Recho, Pierre; Denker, Elsa; Kourakis, Matthew J; Mathiesen, Birthe T; Hannezo, Edouard; Dong, Bo; Jiang, Di
Article Title: Assembly and positioning of actomyosin rings by contractility and planar cell polarity
Affiliation
Abstract: The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells′ anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.
Journal Title: eLife
Volume: 4
Issue OCTOBER2015
ISSN: 2050-084X
Publisher: eLife Sciences Publications  
Date Published: 2015-10-21
DOI: 10.7554/eLife.09206
Open access: no