Glass-like dynamics of collective cell migration Journal Article


Author(s): Angelini, Thomas E; Hannezo, Edouard; Trepatc, Xavier; Marquez, Manuel A; Fredberg, Jeffrey J; Weitz, David A.
Article Title: Glass-like dynamics of collective cell migration
Affiliation
Abstract: Collective cell migration in tissues occurs throughout embryonic development, during wound healing, and in cancerous tumor invasion, yet most detailed knowledge of cell migration comes from single-cell studies. As single cells migrate, the shape of the cell body fluctuates dramatically through cyclic processes of extension, adhesion, and retraction, accompanied by erratic changes in migration direction. Within confluent cell layers, such subcellular motions must be coupled between neighbors, yet the influence of these subcellular motions on collective migration is not known. Here we study motion within a confluent epithelial cell sheet, simultaneously measuring collective migration and subcellular motions, covering a broad range of length scales, time scales, and cell densities. At large length scales and time scales collective migration slows as cell density rises, yet the fastest cells move in large, multicell groups whose scale grows with increasing cell density. This behavior has an intriguing analogy to dynamic heterogeneities found in particulate systems as they become more crowded and approach a glass transition. In addition we find a diminishing self-diffusivity of short-wavelength motions within the cell layer, and growing peaks in the vibrational density of states associated with cooperative cell-shape fluctuations. Both of these observations are also intriguingly reminiscent of a glass transition. Thus, these results provide a broad and suggestive analogy between cell motion within a confluent layer and the dynamics of supercooled colloidal and molecular fluids approaching a glass transition.
Keywords: active matter; Jamming; Cell mechanics; Collective cell dynamics; Nonequilibrium
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 108
Issue 12
ISSN: 1091-6490
Publisher: PNAS  
Date Published: 2011-03-22
Start Page: 4714
End Page: 4719
DOI: 10.1073/pnas.1010059108
Notes: We thank Dr. James Butler and Prof. Johan Mattsson for helpful conversations. This work was supported by the National Science Foundation (NSF) (DMR-1006546) and the Harvard Materials Research Science and Engineering Centers (MRSEC) (DMR-0820484). M.M. gratefully acknowledges partial funding for this work from the University of Malaga (Junta de Andalucia), Project P09-TEP-5369 in collaboration with Prof. Ignacio Loscertales. X.T. acknowledges support of the European Research Council (Starting Grant), the Spanish Ministry of Science and Innovation, and the Instituci├│ Catalana de Recerca i Estudis Avan├žats.
Open access: yes (OA journal)