PATELLINS are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis thaliana Journal Article


Author(s): Tejos, Ricardo; Rodríguez-Furlán, Cecilia; Adamowski, Maciej; Sauer, Michael; Norambuena, Lorena; Friml, Jiří
Article Title: PATELLINS are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis thaliana
Affiliation IST Austria
Abstract: Coordinated cell polarization in developing tissues is a recurrent theme in multicellular organisms. In plants, a directional distribution of the plant hormone auxin is at the core of many developmental programs. A feedback regulation of auxin on the polarized localization of PIN auxin transporters in individual cells has been proposed as a self-organizing mechanism for coordinated tissue polarization, but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport remain unknown. We performed a microarray-based approach to find regulators of the auxin-induced PIN relocation in the Arabidopsis thaliana root. We identified a subset of a family of phosphatidylinositol transfer proteins (PITP), the PATELLINs (PATL). Here, we show that PATLs are expressed in partially overlapping cells types in different tissues going through mitosis or initiating differentiation programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis embryos, primary roots, lateral root primordia, and developing stomata. Higher order patl mutants display reduced PIN1 repolarization in response to auxin, shorter root apical meristem, and drastic defects in embryo and seedling development. This suggests PATLs redundantly play a crucial role in polarity and patterning in Arabidopsis.
Journal Title: Journal of Cell Science
ISSN: 1477-9137
Publisher: Company of Biologists  
Date Published: 2017-08-03
Start Page: Epub ahead of print
Sponsor: ERC-2011 -StG 20101109-PSDP
DOI: 10.1242/jcs.204198
Notes: We are grateful to Prof. Kaye T. Peterman for her kind gift of anti-PATL1 antibodies and Prof. Aliro Villacorta for helpful discussions.
Open access: no