Regulation of Drosophila hematopoietic sites by Activin-β from active sensory neurons Journal Article


Author(s): Makhijani, Kalpana; Alexander, Brandy; Rao, Deepti; Petraki, Sophia; Herboso, Leire; Kukar, Katelyn; Batool, Itrat; Wachner, Stephanie; Gold, Katrina S; Wong, Corinna; O'Connor, Michael B; Brückner, Katja
Article Title: Regulation of Drosophila hematopoietic sites by Activin-β from active sensory neurons
Affiliation IST Austria
Abstract: An outstanding question in animal development, tissue homeostasis and disease is how cell populations adapt to sensory inputs. During Drosophila larval development, hematopoietic sites are in direct contact with sensory neuron clusters of the peripheral nervous system (PNS), and blood cells (hemocytes) require the PNS for their survival and recruitment to these microenvironments, known as Hematopoietic Pockets. Here we report that Activin-β, a TGF-β family ligand, is expressed by sensory neurons of the PNS and regulates the proliferation and adhesion of hemocytes. These hemocyte responses depend on PNS activity, as shown by agonist treatment and transient silencing of sensory neurons. Activin-β has a key role in this regulation, which is apparent from reporter expression and mutant analyses. This mechanism of local sensory neurons controlling blood cell adaptation invites evolutionary parallels with vertebrate hematopoietic progenitors and the independent myeloid system of tissue macrophages, whose regulation by local microenvironments remain undefined.
Journal Title: Nature Communications
Volume: 8
Issue 15990
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2017-07-27
Start Page: Article number: 15990
Copyright Statement: CC BY
URL:
DOI: 10.1038/ncomms15990
Notes: We thank M. Crozatier, S. Diegelmann, C. Evans, C. Han, U. Heberlein, Y.N. Jan, L. Kockel, M. Landgraf, T. Lee, L. Luo, B. Mathey-Prevot, P. Rao, J. Schulte, K. Scott, O. Shafer, K. Sepp, S. Sinenko, S. Younger, M. Zeidler, the Bloomington Stock Center, and the DGRC for antibodies, stocks, plasmids, or sequence information. Special thanks to J. Kronhamn and D. Hultmark for the line HmlΔ-Gal4, UAS-GFP; He-GAL4. K.M. was supported by a HFSP long-term fellowship. K.S.G. was supported by an American Heart Association fellowship. This work was supported by grants from the UCSF Program for Breakthrough Biomedical Research (PBBR), Broad Center, Hellman Foundation, American Cancer Society RSG DDC-122595, American Heart Association 13BGIA13730001, National Science Foundation 1326268, National Institutes of Health 1R01GM112083-01 and 1R56HL118726-01A1 (to K.B.). This investigation was in part conducted in a facility constructed with support from the Research Facilities Improvement Program, Grant number C06-RR16490 from the NCRR/NIH.
Open access: yes (OA journal)