BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana Journal Article

Author(s): Kitakura, Saeko; Adamowski, Maciek; Matsuura, Yuki; Santuari, Luca; Kouno, Hirotaka; Arima, Kohei; Hardtke, Christian S; Friml, Jiří; Kakimoto, Tatsuo; Tanaka, Hirokazu
Article Title: BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana
Affiliation IST Austria
Abstract: Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA), which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been revealed fully. In a previous study, we have identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. Fluorescent proteins tagged BEN3/BIG2 co-localized with markers for TGN / early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA-sensitive and established BEN3/BIG2 as a crucial component of this BFA action at the level of TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF BEN1/MIN7. Taken together our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.
Keywords: Arabidopsis; Auxin; trans-Golgi Network; Brefeldin A; ARF GEF; PIN-FORMED1
Journal Title: Plant and Cell Physiology
ISSN: 1471-9053
Publisher: Oxford University Press  
Date Published: 2017-08-21
Start Page: Article number: pcx118
DOI: 10.1093/pcp/pcx118
Notes: This work has been supported by the Japanese Society for the Promotion of Science (JSPS) KAKENHI [23012026 and 16H04806 to H.T.]; program for Leading Graduate Schools for Osaka University: Interdisciplinary Program for Biomedical Sciences (IPBS) [to Y.M.]; the Central European Institute of Technology [CEIT; CZ.1.05/1.1.00/02.0068]; and the Czech Science Foundation GAČR [GA13-40637S to J.F.]. We thank Ben Scheres, Gerd Jürgens, Glenn R. Hicks, Ian Moore, Kinya Nomura, Natasha Raikhel, Niko Geldner, Qi-Jun Chen and Sheng Yang He for kindly providing published material, Matouš Glanc for technical advice, Yasushi Shiimzu-Mitao for providing a binary vector, and Kayoko Kawamura for technical assistance.
Open access: yes (repository)