Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses Journal Article


Author(s): Chen, Chong; Satterfield, Rachel; Young, Samuel M; Jonas, Peter
Article Title: Triple function of Synaptotagmin 7 ensures efficiency of high-frequency transmission at central GABAergic synapses
Affiliation IST Austria
Abstract: Synaptotagmin 7 (Syt7) is thought to be a Ca2+ sensor that mediates asynchronous transmitter release and facilitation at synapses. However, Syt7 is strongly expressed in fast-spiking, parvalbumin-expressing GABAergic interneurons, and the output synapses of these neurons produce only minimal asynchronous release and show depression rather than facilitation. To resolve this apparent contradiction, we examined the effects of genetic elimination of Syt7 on synaptic transmission at the GABAergic basket cell (BC)-Purkinje cell (PC) synapse in cerebellum. Our results indicate that at the BC-PC synapse, Syt7 contributes to asynchronous release, pool replenishment, and facilitation. In combination, these three effects ensure efficient transmitter release during high-frequency activity and guarantee frequency independence of inhibition. Our results identify a distinct function of Syt7: ensuring the efficiency of high-frequency inhibitory synaptic transmission
Journal Title: Cell Reports
Volume: 21
Issue 8
ISSN: 2211-1247
Publisher: Cell Press  
Date Published: 2017-11-21
Start Page: 2082
End Page: 2089
URL:
DOI: 10.1016/j.celrep.2017.10.122
Notes: We thank Drs. Nils Brose and Magdalena Picher for reading the manuscript. We also thank Alois Schlögl for programming, Florian Marr and Christina Altmutter for technical assistance, Eva Kramberger for manuscript editing, and Michael Schunn, Freyja Langer, and Victoria Wimmer (preclinical facility) for mouse colony management. This project received support from the Austrian Science Fund (FWF P 24909-B24, P.J.), the European Research Council (ERC) under the European Union’s Horizon 2020 Programme for Research and Innovation (692692, P.J.), the Max Planck Society (S.M.Y.), and the National Institute on Deafness and Other Communication Disorders (R01 DC014093; S.M.Y.).
Open access: yes (OA journal)