Gene expression changes of thermo sensitive transient receptor potential channels in obese mice Journal Article


Author(s): Sun, Wuping; Li, Chen; Zhang, Yonghong; Jiang, Changyu; Zhai, Mingzhu; Zhou, Qian; Xiao, Lizu; Deng, Qiwen
Article Title: Gene expression changes of thermo sensitive transient receptor potential channels in obese mice
Affiliation IST Austria
Abstract: Adipose tissues play key roles in energy homeostasis. Brown adipocytes and beige adipocytes in white adipose tissue (WAT) share the similar characters of thermogenesis, both of them could be potential targets for obesity management. Several thermo-sensitive transient receptor potential channels (thermoTRPs) are shown to be involved in adipocyte biology. However, the expression pattern of thermoTRPs in adipose tissues from obese mice is still unknown. The mRNA expression of thermoTRPs in subcutaneous WAT (sWAT) and interscapular brown adipose tissue (iBAT) from lean and obese mice were measured using reverse transcriptase-quantitative PCRs (RT-qPCR). The results demonstrated that all 10 thermoTRPs are expressed in both iBAT and sWAT, and without significant difference in the mRNA expression level of thermoTRPs between these two tissues. Moreover, Trpv1 and Trpv3 mRNA expression levels in both iBAT and sWAT were significantly decreased in high fat diet (HFD)-induced obese mice and db/db (leptin receptor deficient) mice. Trpm2 mRNA expression level was significantly decreased only in sWAT from HFD-induced obese mice and db/db mice. On the other hand, Trpv2 and Trpv4 mRNA expression levels in iBAT and sWAT were significantly increased in HFD-induced obese mice and db/db mice. Taken together, we conclude that all 10 thermoTRPs are expressed in iBAT and sWAT. And several thermoTRPs differentially expressed in adipose tissues from HFD-induced obese mice and db/db mice, suggesting a potential involvement in anti-obesity regulations.
Journal Title: Cell Biology International
Volume: 41
Issue 8
ISSN: 1095-8355
Publisher: Wiley-Blackwell  
Date Published: 2017-08-01
Start Page: 908
End Page: 913
DOI: 10.1002/cbin.10783
Notes: We are grateful to Dr. Makoto Tominaga (National Institute for Physiological Sciences of Japan) for his help. This work was supported by grants from Shenzhen Municipal Science, Technology and Innovation Commission (No. JCYJ20160429182122843, No. JCYJ20160429181451546), Municipal Key Laboratory Program of Shenzhen (No. ZDSYS20140509150415945).
Open access: no