Roles of afadin in functional differentiations of hippocampal mossy fiber synapse Journal Article


Author(s): Geng, Xiaoqi; Maruo, Tomohiko; Mandai, Kenji; Supriyanto, Irwan; Miyata, Muneaki; Sakakibara, Shotaro; Mizoguchi, Akira; Takai, Yoshimi; Mori, Masahiro
Article Title: Roles of afadin in functional differentiations of hippocampal mossy fiber synapse
Affiliation IST Austria
Abstract: A hippocampal mossy fiber synapse has a complex structure and is implicated in learning and memory. In this synapse, the mossy fiber boutons attach to the dendritic shaft by puncta adherentia junctions and wrap around a multiply-branched spine, forming synaptic junctions. We have recently shown using transmission electron microscopy, immunoelectron microscopy and serial block face-scanning electron microscopy that atypical puncta adherentia junctions are formed in the afadin-deficient mossy fiber synapse and that the complexity of postsynaptic spines and mossy fiber boutons, the number of spine heads, the area of postsynaptic densities and the density of synaptic vesicles docked to active zones are decreased in the afadin-deficient synapse. We investigated here the roles of afadin in the functional differentiations of the mossy fiber synapse using the afadin-deficient mice. The electrophysiological studies showed that both the release probability of glutamate and the postsynaptic responsiveness to glutamate were markedly reduced, but not completely lost, in the afadin-deficient mossy fiber synapse, whereas neither long-term potentiation nor long-term depression was affected. These results indicate that afadin plays roles in the functional differentiations of the presynapse and the postsynapse of the hippocampal mossy fiber synapse.
Journal Title: Genes to Cells
Volume: 22
Issue 8
ISSN: 1365-2443
Publisher: Wiley-Blackwell  
Date Published: 2017-01-01
Start Page: 715
End Page: 722
DOI: 10.1111/gtc.12508
Notes: We thank Noriko Hattori and Yu Itoh for technical assistance. This study was supported by MEXT KAKENHI Grant Numbers 26114007 (to Y.T.), 16H06461 (to A.M.) and 16H06463 (to K.M.); and JSPS KAKENHI Grant Numbers 26251013 (to Y.T.) and 16K19036 (to T.M.); AMED SEEds B program Grant Number B25 (to A.M.).
Open access: no