Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation Journal Article


Author(s): Krens, Gabriel; Veldhuis, Jim H; Barone, Vanessa; Čapek, Daniel; Maître, Jean-Léon; Brodland, Wayne; Heisenberg, Carl-Philipp
Article Title: Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation
Affiliation IST Austria
Abstract: The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation.
Keywords: Tissue Surface Tension; Gastrulation; Zebrafish; cell internalization
Journal Title: Development
Volume: 144
Issue 10
ISSN: 1477-9129
Publisher: Company of Biologists  
Date Published: 2017-05-15
Start Page: 1798
End Page: 1806
Sponsor: Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant (RGPIN-36514 to G.W.B.), Austrian Science Foundation (FWF) (I930-B20 to C.-P.H.)
URL:
DOI: 10.1242/dev.144964
Notes: We thank D. Wagner for providing the pky zebrafish line, D. Gilmour, E. Raz and T. Kudoh for providing plasmids, and E. Paluch, D. Siekhaus and M. Sixt for reading earlier versions of the manuscript. We are grateful to all members of the Heisenberg and Sixt groups for fruitful discussions, to M. Sikora, J. Guzman and S. Pallarés for help with the analysis of the AFM data, measuring trans-epithelial resistance and measuring IF osmolarity, respectively; and the zebrafish, light microscopy and mechanical workshop facilities at IST Austria and MPI-CBG for assistance.
Open access: yes (repository)