Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses Journal Article


Author(s): Dimitrov, Dimitar; Guillaud, Laurent; Eguchi, Kohgaku; Takahashi, Tomoyuki
Article Title: Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses
Affiliation IST Austria
Abstract: Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.
Keywords: Synaptic Vesicles; Calyx of held; capacitance measurements; giant synapse; neuronal cell culture; neurotrophin-3; synapse imaging
Journal Title: Methods in Molecular Biology
Volume: 1727
ISSN: 1064-3745
Publisher: Springer  
Date Published: 2018-01-01
Start Page: 201
End Page: 215
DOI: 10.1007/978-1-4939-7571-6_15
Open access: no