Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls Journal Article


Author(s): Li, Lanxin; Krens, Gabriel; Fendrych, Matyas; Friml, Jiří
Article Title: Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls
Affiliation IST Austria
Abstract: The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.
Keywords: Auxin signaling; cell wall pH; cell elongation, Hypocotyl; live-cell imaging
Journal Title: Bio-protocol
Volume: 8
Issue 1
ISSN: 2331-8325
Publisher: Bio-protocol LLC  
Publication Place: United States
Date Published: 2018-01-05
Start Page: Article number: e2685
Copyright Statement: CC BY 4.0
URL:
DOI: 10.21769/BioProtoc.2685
Notes: This protocol was adapted from Fendrych et al., 2016. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian Science Fund (FWF) [M 2128-B21].
Open access: yes (OA journal)