A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis Journal Article

Author(s): Adamowski, Maciek; Narasimhan, Madhumitha; Kania, Urszula; Glanc, Matous; De Jaeger, Geert; Friml, Jiří
Article Title: A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis
Affiliation IST Austria
Abstract: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterised compared to that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologues of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2) loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the on-going characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in A. thaliana.
Journal Title: The Plant Cell
Volume: 30
ISSN: 1532-298X
Publisher: American Society of Plant Biologists  
Publication Place: United States
Date Published: 2018-01-01
Start Page: 700
End Page: 716
Copyright Statement: (c) 2018 American Society of Plant Biologists. All rights reserved.
Sponsor: EC | European Research Council (ERC) 282300 Jiří Friml; Ministry of Education of the Czech Republic NPUI - LO1417 Matouš Glanc
DOI: 10.1105/tpc.17.00785
Open access: yes (OA journal)