A biochemical network controlling basal myosin oscillation Journal Article


Author(s): Qin, Xiang; Hannezo, Edouard; Mangeat, Thomas; Liu, Chang; Majumder, Pralay; Liu, JJiaying; Choesmel Cadamuro, Valerie; McDonald, Jocelyn A; Liu, Yinyao; Yi, Bin; Wang, Xiaobo
Article Title: A biochemical network controlling basal myosin oscillation
Affiliation IST Austria
Abstract: The actomyosin cytoskeleton, a key stress-producing unit in epithelial cells, oscillates spontaneously in a wide variety of systems. Although much of the signal cascade regulating myosin activity has been characterized, the origin of such oscillatory behavior is still unclear. Here, we show that basal myosin II oscillation in Drosophila ovarian epithelium is not controlled by actomyosin cortical tension, but instead relies on a biochemical oscillator involving ROCK and myosin phosphatase. Key to this oscillation is a diffusive ROCK flow, linking junctional Rho1 to medial actomyosin cortex, and dynamically maintained by a self-activation loop reliant on ROCK kinase activity. In response to the resulting myosin II recruitment, myosin phosphatase is locally enriched and shuts off ROCK and myosin II signals. Coupling Drosophila genetics, live imaging, modeling, and optogenetics, we uncover an intrinsic biochemical oscillator at the core of myosin II regulatory network, shedding light on the spatio-temporal dynamics of force generation.
Keywords: Myosin; morphogenesis; Computational biophysics
Journal Title: Nature Communications
Volume: 9
Issue 1
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2018-03-23
Start Page: Article number: 1210
Copyright Statement: CC BY
URL:
DOI: 10.1038/s41467-018-03574-5
Notes: We thank Adam Martin, Yohanns Bellaiche, Daniel Kiehart, Bloomington Drosophila stock center and Vienna Drosophila RNAi center for flies. We thank Change Tan for MBS antibody. We thank Tri Toulouse platform for imaging acquisition advice. We thank Francois Schweisguth and Karine Belguise for discussion of manuscript preparation. This work was supported by the Institut National de la Santeet de la Recherche Medicale [the ATIP-Avenir program (2012 – 2016)]; Region Midi-Pyrenees Excellence program (2013 – 2016); the National Natural Science Foundation of China (grant number 81670552 to B.Y., 11772088, 31470906 to Y.L.); the National Science Foundation of USA (grant number NSF 1456053 to J.A.M.); and the Indian Foundation (DST [SB/YS/LS-88/ 2014] and UGC [MRP-MAJOR-ZOOL-2013-16812] to P.M.
Open access: yes (OA journal)