Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration Journal Article


Author(s): Ratheesh, Aparna; Biebl, Julia; Smutny, Michael; Vesela Jana; Papusheva, Ekaterina; Krens, Gabriel; Kaufmann, Walter; György, Attila; Casano, Alessandra M; Siekhaus, Daria E
Article Title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage invasive migration
Affiliation IST Austria
Abstract: Migrating cells penetrate tissue barriers during development, inflammatory responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally confined environments requires changes in the mechanical properties of the surrounding cells using embryonic Drosophila melanogaster hemocytes, also called macrophages, as a model. We find that macrophage invasion into the germband through transient separation of the apposing ectoderm and mesoderm requires cell deformations and reductions in apical tension in the ectoderm. Interestingly, the genetic pathway governing these mechanical shifts acts downstream of the only known tumor necrosis factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald. Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated tight junction protein). We therefore elucidate a distinct molecular pathway that controls tissue tension and demonstrate the importance of such regulation for invasive migration in vivo.
Keywords: Myosin; hemocyte; plasmatocyte; immune cell; stiffness; Eiger; Patj; Crumbs; invasion; infiltration
Journal Title: Developmental Cell
Volume: 45
Issue 3
ISSN: 1534-5807
Publisher: Elsevier  
Date Published: 2018-05-07
Start Page: 331
End Page: 346
DOI: 10.1016/j.devcel.2018.04.002
Notes: D.S. was supported by Marie Curie CIG 34077/IRTIM and A.R. by Marie Curie IIF GA-2012-32950 BB: DICJI. A.R. and A.G. were also supported by the Austrian Science Foundation (FWF): P_DASI_FWF01_P29638. We are deeply grateful to Ruth Lehmann of NYU School of Medicine Skirball Center in whose lab the work underlying this research was begun.
Open access: no