Lymphatic exosomes promote dendritic cell migration along guidance cues Journal Article

Author(s): Brown, Markus; Johnson, Louise A; Leone, Dario A; Májek, Peter; Vaahtomeri, Kari; Senfter, Daniel; Bukosza, Nora; Schachner, Helga; Asfour, Gabriele; Langer, Brigitte; Hauschild, Robert; Parapatics, Katja; Hong, Young-Kwon; Bennett, Keiryn L; Kain, Renate; Detmar, Michael; Sixt, Michael; Jackson, David G; Kerjaschki, Dontscho
Article Title: Lymphatic exosomes promote dendritic cell migration along guidance cues
Affiliation IST Austria
Abstract: Lymphatic endothelial cells (LECs) release extracellular chemokines to guide the migration of dendritic cells. In this study, we report that LECs also release basolateral exosome-rich endothelial vesicles (EEVs) that are secreted in greater numbers in the presence of inflammatory cytokines and accumulate in the perivascular stroma of small lymphatic vessels in human chronic inflammatory diseases. Proteomic analyses of EEV fractions identified > 1,700 cargo proteins and revealed a dominant motility-promoting protein signature. In vitro and ex vivo EEV fractions augmented cellular protrusion formation in a CX3CL1/fractalkine-dependent fashion and enhanced the directional migratory response of human dendritic cells along guidance cues. We conclude that perilymphatic LEC exosomes enhance exploratory behavior and thus promote directional migration of CX3CR1-expressing cells in complex tissue environments.
Journal Title: Journal of Cell Biology
Volume: 217
Issue 6
ISSN: 0021-9525
Publisher: Rockefeller University Press  
Date Published: 2018-06-01
Start Page: 2205
End Page: 2221
DOI: 10.1083/jcb.201612051
Open access: yes (OA journal)