Rapid and reversible root growth inhibition by TIR1 auxin signalling Journal Article


Author(s): Fendrych, Matyas; Akhmanova, Maria; Merrin, Jack; Glanc, Matous; Hagihara Shinya; Takahashi Koji; Uchida Naoyuki; Torii Keiko U; Friml, Jiří
Article Title: Rapid and reversible root growth inhibition by TIR1 auxin signalling
Affiliation IST Austria
Abstract: The phytohormone auxin is the information carrier in a plethora of developmental and physiological processes in plants(1). It has been firmly established that canonical, nuclear auxin signalling acts through regulation of gene transcription(2). Here, we combined microfluidics, live imaging, genetic engineering and computational modelling to reanalyse the classical case of root growth inhibition(3) by auxin. We show that Arabidopsis roots react to addition and removal of auxin by extremely rapid adaptation of growth rate. This process requires intracellular auxin perception but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA co-receptor complex is required for the growth regulation, hinting to a novel, non-transcriptional branch of this signalling pathway. Our results challenge the current understanding of root growth regulation by auxin and suggest another, presumably non-transcriptional, signalling output of the canonical auxin pathway.
Journal Title: Nature Plants
Volume: 4
Issue 7
ISSN: 2055-0278
Publisher: Springer  
Publication Place: England
Date Published: 2018-06-25
Start Page: 453
End Page: 459
Copyright Statement: M.F. was supported by the Austrian Science Fund (FWF) (M 2128-B21), and in the final stages of the project by the Czech Science Foundation (18-10116Y). M.A. was supported by the Austrian Science Fund (FWF) (M2379-B28). Additional funding for M.G. was received from the Ministry of Education of the Czech Republic/MŠMT project NPUI-LO1417. N.U. received MEXT/JSPS KAKENHI JP16H01462, S.H. received JP17H06350, K.T. received JP26440140 and K.U.T. received JP16H01237. K.U.T. is supported by GBMF Grant No. 3035. S.H. is a JST PRESTO Investigator and K.U.T. is an HHMI-GBMF Investigator. J.F. acknowledges the ERC Advanced Grants (ETAP).
DOI: 10.1038/s41477-018-0190-1
Open access: no